In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy

Abstract

Aim: In-Vitro Anti-inflammatory Activity of Methanolic Extract of Convolvulus pluricaulis Choisy.
Material & Methods- The whole plant parts of Convolvulus pluricaulis Choisy were purchased from the local market. Whole plant materials were dried under shade and subjected to coarse powder for extraction process. Accurately weighed quantity of whole plant material was extracted using 95 % methanol by soxhlet apparatus for 72 h. Qualitative chemical tests of methanolic extracts were subjected to various chemical tests to detect various phytoconstituents. Solvent systems ethyl acetate: methanol: water (77:13:10) were found to be most satisfactory solvent system. After development of plates, they were air-dried and number of spots, color and Rf values were recorded. The % heamolysis was calculated by assuming the heamolysis produced by the control group as 100 %.
Results: The preliminary phytochemical analysis revealed that different active constituent present in different extracts such as carbohydrates, proteins, amino acids, fat, oils, steroids, terpenoids, glycosides, alkaloids, tannins and other phenolics compounds. At a concentration of 500 µg/ml, the extract produced 71.59% protection of RBC haemolysis as compared with 72.73% produced by prednisolone.  The methanolic extract of selected plant showed 39.70% inhibition. The Diclofenac sodium showed 55.88 % inhibition against denaturation of protein.
Conclusion: In conclusion, it can be stated that the methanolic extract has beneficial effects in long lasting in membrane stabilizing method, inhibition of protein denaturation method and proteinase model.
Keywords: In-Vitro, Anti-inflammatory Activity, Methanolic Extract, Convolvulus pluricaulis Choisy, Protein Denaturation Method