A.Hanumappa Chowdary
M.PHARM. (PHARMACEUTICAL ANALYSIS) JNTUA, OTPRI, Ananthapuramu
R. Kiranjyothi
M.PHARM. (PHARMACEUTICAL ANALYSIS) JNTUA, OTPRI, Ananthapuramu
J. Priyanka
M.PHARM. (PHARMACEUTICAL ANALYSIS) JNTUA, OTPRI, Ananthapuramu
Abstract
A simple, rapid and accurate RP-HPLC method was developed for the quantification of Cefixim in bulk and its formulation by RP-HPLC method using C18 ODS column (Phenomenex Luna, 250 x 4.6 mm, 5μm) in isocratic mode. The mobile phase consisted of methanol and 0.1% Orthophosphoric acid in the ratio of 70:30 (v/v) water: methanol was used. The flow rate was maintained at 1.0 mL/min and the injection volume was 20μL. Detection wavelength with UV detector at 289 nm and run time was kept 10 min. The retention time of Cefixim was 3.654 min. The method was linear over the concentration range50 -150μg/ml. The recovery was found to be 99.11 %. The validation of method was carried out utilizing ICH-guidelines. The described HPLC method was successfully employed for the analysis of pharmaceutical formulations.
Key words: HPLC method, Cefixim, Method development, ICH-guidelines