Mrunal K. Shirsat
faculty of Pharmacy, Pacific University Udaipur Rajasthan
Siju V. Mathew
Research Scholar, Department of Phamacognosy, Pacific University Udaipur Rajasthan
Abstract
The plants aerial parts of Indigofera astragalin was selected for the present studies. Fresh and mature plant plants of I. astragalina were collected from tiruchengode, Tamilnadu. The plant was authenticated by Dr. G.V.S. Murthy, Joint Director, Botanical Survey of India, Coimbatore, tamilnadu, India. A voucher specimen is preserved in our laboratory for future reference (Voucher No.: PCIAS 007). The in vitro methods include the determination of Total phenolic content and Total flavonoid content. Besides these, the antioxidant activity of the extracts and fractions were determined in different in-vitro experimental methods like Diphenyl-picryl-hydrazyl (DPPH) radical, Superoxide free radical (O2•−), Peroxide radical (H2O2), ABTS radical cation scavenging activity and Nitric oxide (NO) free radical scavenging activity with reference to standard antioxidant ascorbic acid. The capabilities of ethanolic and aqueous extracts of aerial parts of Indigofera astragalin to scavenge DPPH were measured in-vitro the related IC50 values and the % scavenging. The chloroform fraction of Indigofera astragalin was found to be an effective superoxide anion scavenger to scavenge the superoxide anions as compared to ascorbic acid which is measured in terms of IC50 (87.76 μg/ml) were obtained.
Key words: Anti-oxidant, I. astragalina, DPPH, Superoxide free radical, ABTS