SYNTHESIS, CHARACTERIZATION AND ANTI-MICROBIAL ACTIVITY OF 3-SUBSTITUTED 1H-INDOLE-2,3-DIONE ANALOGUES

Abstract

Antimicrobial drugs have greatest contribution to therapeutics they are one of the few curative drugs. Antibiotics are the substances produced by microorganisms, which suppress the growth of or kill other microorganisms at very low concentrations. All chemicals were provided from our college. All solvents were redistilled before use. Reactions were routinely monitored by thin layer chromatography and spots were visualized by exposure to iodine vapour or UV light. All the synthesized compounds were purified by recrystallization. Melting points were determined by using open capillary method. and equimolar amines and 5-Nitro (R=NO2) substituted isatins (1 mmol of each) were added to 96% w/w ethanol (20 mL) containing 8 drops of glacial acetic acid. The mixture was heated under reflux for 5 h and then cooled to room temperature. Antibacterial activities was test on nutrient medium against, Staphylococcus aureus (MTTC 4325), and Escherchia coli (MTTC 3265), which are representative types of gram positive and gram negative organisms respectively. The antibacterial activities of the compounds were assessed by disc-diffusion method. Compound IIIc and IIId shown excellent antibacteial against E.coli and S.Aureus bacteia, range from 20 to 24 nearly significant to standard drug amoxicilline, which shown 24 to 25mm on different two concentration i.e. 50 and 100 μg/ml, the substitution of phenol ring, pyridine ring at 3-substituted 1H-indole-2,3-dione analogues having responsible for significant antibacterial activity.
Keywords: 1H-indole-2,3-dione; Isatin; Antibacteial activity; Disc diffusion method.