Sachin Bhusari
University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
Vikas Bhise
University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
Pravin Wakte
University Department of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
Abstract
A simple, precise and cost effective UV- visible spectrophotometric method has been developed and validated according to the guidelines of the International Conference on Harmonization (ICH Q2 (R1) for the estimation of resveratrol in bulk and formulation. Spiked resveratrol solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of resveratrol were prepared. Calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters viz. linearity, accuracy, precision, robustness, ruggedness, limit of detection and limit of quantitation were calculated. The maximum wavelength of resveratrol was found to be 215 nm. The correlation coefficient over the concentration range of 0.5-6 μg/ml was found to be 0.999. Developed UV method was found to be precise for the intra-day and inter-day study and shows percent relative standard deviation in the range of 0.58 & 1.20 to 0.28 to 1.08 respectively. The total percent recovery of resveratrol was found to be 98.18 %. A simple, precise and cost effective UV- visible spectrophotometric method was developed and validated as per ICH guidelines. The results suggest that the developed method was found to be robust and it can be applicable in routine analysis and efficiently used for the estimation of resveratrol in bulk as well as combined dosage form.
Keywords: Resveratrol, HPLC method development, Validation