To Develop Reverse Phase High Performance Liquid Chromatography Method for Simultaneous Estimation of Atenolol and Indapamide in Pharmaceutical Formulation

Abstract

An economical RP-HPLC method were developed and validated for the simultaneous estimation of Atenolol (ATL) and Indapamide (INDP) in combined tablet dosage form which has been accurate, precise and simple RP-HPLC methods. The RP-HPLC method uses a Shimadzu LC 10 ATVP system with Luna C18 column and Methanol: Acetonitrile and Water (pH 3.5 adjusted with orthophsophoric acid) in the ratio of 45:25:30 (v/v) as the mobile phase. The estimation was performed at 226 nm using a diode array detector as all the two analyte absorbed well at this wavelength. The flow rate was maintained at 1.2 ml min-1.  Linearity of chromatographic method was found in the concentration range of 2-10 and 5–25 µg/mL for ATL and INDP respectively. The recoveries were in the range of 99.75 ± 0.0642% and for ATL, 99.71 ± 0.189 % for INDP in HPLC methods respectively. The method was linear in concentration ranges were found to be within the limit and result of accuracy was found to be between 98 to 101%. The method was precise as the % RSD was found that NMT 2.0% and specificity studies reveal no interference in the standard peaks due to placebo. The developed precise method was validated in respect to linearity, range, precision, accuracy, robustness.
Keywords: linearity, validation, Accuracy, Precision