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Dr. Charanya
MDS, Senior Lecturer, Department of Conservative Dentistry and Endodontics, Tagore Dental College and Hospital, Rathinamangalam, Chennai-600127
Dr.S. Balagopal
MDS, M.Sc, Professor & HOD, Department of Conservative Dentistry and Endodontics, Tagore Dental College and Hospital, Rathinamangalam, Chennai-600127
Dr. Vandana James
MDS, Reader, Department of Conservative Dentistry and Endodontics, Tagore Dental College and Hospital, Rathinamangalam, Chennai-600127
Dr. S. Navapriya
BDS, Department of Conservative Dentistry and Endodontics, Tagore Dental College and Hospital, Rathinamangalam, Chennai-600127
Dr. D. Mohanraj
BDS, Department of Conservative Dentistry and Endodontics, Tagore Dental College and Hospital, Rathinamangalam, Chennai-600127
Abstract
Introduction: Enterococcus faecalis is a gram-positive anaerobic bacteria highly virulent in nature that can cause reinfection in root canal treated tooth. Calcium hydroxide with oil-based vehicles are also used as intracanal medicament. Oils such as thyme oil, basil oil, oregano oil have proved antibacterial effect.
Aim: The aim of this study is to assess the antimicrobial efficacy of calcium hydroxide mixed with oregano oil, thyme oil, basil oil as vehicle against E.faecalis.
Materials and Methods: Thirty muller Hinton agar plates were taken as sample and were grouped into 5 groups as Group 1 - oregano oil with Calcium hydroxide, Group 2- Thyme oil with Calcium hydroxide, Group 3 - Basil oil with Calcium hydroxide, Group 4 - Calcium hydroxide with saline, Group 5 – Negative control. The agar plates were inoculated with strains of E.faecalis (ATCC29212). Agar wells were created and test intracanal medicaments were placed in agar wells in group 1-4 where group 5 was used as negative control. The agar plates were incubated at 37 0C for 72 hours. After incubating, the agar plates were examined for zone of inhibition for assessing the degree of susceptible or resistance of test organism.
Statistical analysis: Statistical analysis was done with one way anova/ kruskal wallis test and post hoc bonferroni test and was statistically significant (P <0.05) Result: It was shown that Thyme oil (26±0.00) was more effective in eradicating Enterococcus faecalis when compared to Basil oil (14.25 ± 0.5) and Oregano oil (23.75 ± 0.5).
Conclusion: Thyme oil with calcium hydroxide has significantly high antimicrobial efficacy against Enterococcus faecalis.
Key Messages: Enterococcus faecalis is a gram-positive anaerobic bacteria highly virulent in nature that can cause reinfection in root canal treated tooth. Calcium hydroxide with oil-based vehicles is also used as intracanal medicament. Oils such as thyme oil, basil oil, and oregano oil have proved antibacterial effect. Thyme oil with calcium hydroxide has significantly high antimicrobial efficacy against Enterococcus faecalis.