Preparation and Evaluation of Wrightia Tinctoria Extract Transdermal Patch and Antimicrobial Activity against Clinical Pathogenic Microorganisms

Abstract

Investigate phytoconstituents in chloroform leaf extracts of Wrightia tinctoria while developing transdermal patches. Transdermal patches of Wrightia tinctoria herbal extract prepared with a solvent casting technique. Based on a physicochemical and in vitro drug diffusion investigation, numerous formulation parameters, drug-polymer ratios, and permeation enhancers were optimized, with the optimum formulation chosen for optimisation. The most effective formulation will be tested for antibacterial activity. This demonstrates the drug's uniform dispersion during the polymer ratio patch 1:4. Information gathered from the in-vitro diffusion profile chosen for formulation development. With varied reaction kinetics, the greater correlation coefficients (r2) indicate greater comprehension of the product diffusion and diffusion rate mechanism. These findings imply that drug release from this patch was controlled by diffusion. Based on in vitro diffusion and physicochemical investigations, 1:4 ratios (formulation 2) were determined to be the optimal formulation. Antimicrobial activity analysis was then performed on Formulation 2. The antimicrobial test findings proved that the patch effectively reduced bacteria growth. The results reveal that the Wrightia tinctoria plant chloroform extract has antibacterial action against Staphylococcus aureus.
Keywords: Wrightia tinctoria, Transdermal patch, antimicrobial studies.