INHIBITION OF NITRIC OXIDE AND PROINFLAMMATORY CYTOKINES BY AQUEOUS EXTRACT OF TERMINALIA ARJUNA IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS

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Amit Gupta

Abstract

Despite its beneficial role in host defense mechanisms, enhancement of nitric oxide (NO) production and pro-inflammatory cytokines from human peripheral blood mononuclear cells has been implicated in several inflammatory diseases. To clarify the mechanisms of the inhibition of NO as well as pro-inflammatory cytokines from the aqueous extract of stem bark of Terminalia arjuna, we evaluated whether the aqueous extract could modulate the production of NO and pro-inflammatory cytokines from human peripheral blood mononuclear cells. The results indicate that the aqueous extract of Terminalia arjuna were effective inhibitors of lipolysaccharide (LPS)-induced NO and pro-inflammatory cytokines in human peripheral blood mononuclear cells. To test the inhibitory effects of aqueous extract of Terminalia arjuna on Th1 type of cytokines, we performed ELISA assays for TNF-alpha and IFN-gamma in LPS-stimulated human peripheral blood mononuclear cells. In these assays, aqueous extract of Terminalia arjuna produced dose-dependent decreases in the production of TNF-alpha and IFN-gamma.
Key words: Terminalia arjuna, nitric oxide, PBMC, lipopolysaccharide

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How to Cite
Gupta, A. (2016). INHIBITION OF NITRIC OXIDE AND PROINFLAMMATORY CYTOKINES BY AQUEOUS EXTRACT OF TERMINALIA ARJUNA IN HUMAN PERIPHERAL BLOOD MONONUCLEAR CELLS. International Journal of Pharmaceutical and Biological Science Archive, 2(8). Retrieved from http://ijpba.in/index.php/ijpba/article/view/40
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Research Article