Dipak Thange
Research scholar, Department of Pharmacy, Sunrise University, Alwar Rajasthan.
Arun Sharma
Research supervisor, Department of Pharmacy, Sunrise University, Alwar Rajasthan.
Sheetal Kumar Bhong
Department of Chemistry, Sinhgad College of Science, Pune 43.
Abstract
The objective is to create a liquid chromatography-mass spectrometry approach that is fast, precise, reliable, and effective. This method will be validated according to the requirements set by the International Council for Harmonisation (ICH). The purpose of this method is to simultaneously measure the levels of apigenin and luteolin in Achillea magna. The chromatographic separation was accomplished using a C8 column with dimensions of 150 x 4.6mm i.d. and a 5µ Hibar Lichrospher. The mobile phase consisted of a mixture of 0.1% formic acid and acetonitrile in a ratio of 20:80 v/v. The volumetric flow rate was 0.4 millilitres per minute. The apigenin and luteolin compounds exhibited retention times of 8.48 min and 8.01 min, respectively. Apigenin and luteolin demonstrated a linear relationship within the concentration range of 10-50 ng/ml. The technique was verified for criteria such as system appropriateness, specificity, linearity, accuracy, precision, limit of detection, and limit of quantification. The suggested technique was effectively used to simultaneously estimate the elements in Achillea magna and build a quantitative approach for determining both apigenin and luteolin from Achillea magna.
KEYWORDS: LC-MS method, Achillea magna, simultaneous estimation, flavonoids, apigenin, luteolin.